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  • I have my protein complex in a buffer with very high salt concentration plus 5% glycerol. Is this buffer compatible with mass spectrometry?

Are there any good papers describing phosphoproteomics?

Yes! See the publication linked here.

Publications

  • Dephoure N, Gould KL, Gygi SP, Kellogg DR "Mapping and analysis of phosphorylation sites: a quick guide for cell biologists." Mol Biol Cell, 2013, 24(5) p. 535-42.
    Summary | Full Text (PMC) | Full Text (DOI)

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