Can I submit a silver-stained gel?

Yes, if it is stained by mass spectrometry-compatible silver stain protocol.  Many commercial vendors sell such kits.


  • Gharahdaghi F, Weinberg CR, Meagher DA, Imai BS, Mische SM "Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity." Electrophoresis, 1999, 20(3) p. 601-5.
    Summary | Full Text (DOI)

  • Aurandt J, Vikis HG, Gutkind JS, Ahn N, Guan KL "The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG." Proc Natl Acad Sci U S A, 2002, 99(19) p. 12085-90.
    Summary | Full Text (PMC) | Full Text (DOI)

  • Web Links

    Posted in Sample Preparation