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  • I have my protein complex in a buffer with very high salt concentration plus 5% glycerol. Is this buffer compatible with mass spectrometry?

What database is used for the searches? Are databases updated regularly?

Our default database is UniProt.  As routine policy, for poorly annotated proteomes we search both the sections of Uniprot i.e. SwissProt (curated) and Trembl (non-curated).  After both the searches, non redundant protein list with each IDs supported by at least one unique peptide is submitted to the user. Both databases are updated every month.

Posted in Bioinformatics