• Home
  • Technology
  • Technical FAQs
  • Sample Preparation
  • I have my protein complex in a buffer with very high salt concentration plus 5% glycerol. Is this buffer compatible with mass spectrometry?

What is a false discovery rate (FDR) ?

The reporting of a FDR has become mandatory.  The FDR is essentially a statistical and computational measure to establish the certainty of the protein identification in a given dataset. There are several methods, we typically use a decoy database to measure the false discovery rate.


  • Elias JE, Gygi SP "Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry." Nat Methods, 2007, 4(3) p. 207-14.
    Summary | Full Text (DOI)

  • Keller A, Nesvizhskii AI, Kolker E, Aebersold R "Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search." Anal Chem, 2002, 74(20) p. 5383-92.
    Summary | Full Text (DOI)

  • Posted in Bioinformatics