Co-immunoprecipitation (Co-IP) is a popular technique to identify physiologically relevant protein-protein interactions by using target protein-specific antibodies to indirectly capture proteins that are bound to a specific target protein. These protein complexes can then be analyzed to identify new binding partners, binding affinities, the kinetics of binding and the function of the target protein.

Related Publications

Brymora A, Valova VA, Robinson PJ “Protein-protein interactions identified by pull-down experiments and mass spectrometry.” Curr Protoc Cell Biol, 2004, Chapter 17() p. Unit 17.5.

Summary | Full Text (DOI)

Berggård T, Linse S, James P “Methods for the detection and analysis of protein-protein interactions.” Proteomics, 2007, 7(16) p. 2833-42.

Summary | Full Text (DOI)

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Instruments Used

Orbitrap Elite

Q Exactive HF