The biotin ligase, BirA* is fused to a target of interest, cells can then be treated with biotin and targeted proteins can be identified using standard “biotin-avidin” capture techniques.

Related Publications

Roux KJ, Kim DI, Burke B “BioID: a screen for protein-protein interactions.” Curr Protoc Protein Sci, 2013, 74() p. 19.23.1-19.23.14.

Summary | Full Text (DOI)

Kim DI, Birendra KC, Zhu W, Motamedchaboki K, Doye V, Roux KJ “Probing nuclear pore complex architecture with proximity-dependent biotinylation.” Proc Natl Acad Sci U S A, 2014, 111(24) p. E2453-61.

Summary | Full Text (PMC) | Full Text (DOI)


Instruments Used

Orbitrap Elite

Q Exactive HF