The biotin ligase, BirA* is fused to a target of interest, cells can then be treated with biotin and targeted proteins can be identified using standard “biotin-avidin” capture techniques.
Roux KJ, Kim DI, Burke B “BioID: a screen for protein-protein interactions.” Curr Protoc Protein Sci, 2013, 74() p. 19.23.1-19.23.14.
Kim DI, Birendra KC, Zhu W, Motamedchaboki K, Doye V, Roux KJ “Probing nuclear pore complex architecture with proximity-dependent biotinylation.” Proc Natl Acad Sci U S A, 2014, 111(24) p. E2453-61.
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Q Exactive HF