Top Down Antibody Profiling

Antibody analysis and profiling is a hot topic within the field of proteomics. With our Orbitrap-based mass spectrometers and custom methods, we can provide relative quantitation of antibodies and tandem MS information to confirm its sequence.

We are able to profile mass differences on an intact antibody (and to count the number of modifications). Typical amounts of material required are 50 µL of a 2 pmol/µL solution of each antibody dialyzed against 100 mM ammonium acetate overnight at 4°C.

Related Publications

Fornelli L, Damoc E, Thomas PM, Kelleher NL, Aizikov K, Denisov E, Makarov A, Tsybin YO “Analysis of intact monoclonal antibody IgG1 by electron transfer dissociation Orbitrap FTMS.” Mol Cell Proteomics, 2012, 11(12) p. 1758-67.

Summary | Full Text (PMC) | Full Text (DOI)


Instruments Used

Orbitrap Elite

Q Exactive